The cell interior is a different world that we are only starting to explore. Though there are a number of approaches for examining the inner workings of the cell, the reductionist approach of building up complexity attracts many with physical science and engineering backgrounds. Building a Cell from its Component Parts, (PDF) spans a range of spatial scales from single protein molecules to vesicle and cell-sized structures capable of complicated behaviors. Contributions include; reconstituting membrane fission and fusion; methods to combine cytoskeletal assemblies with fabricated devices such as chambers or pillar arrays; methods for combining different motors and cytoskeletal components in defined ways to produce more complex behaviors; reconstituting important biological processes that normally take place on membrane surfaces; and procedures for encapsulating protein machines within vesicles or droplets.
NOTE: The product only includes the ebook, Building a Cell from its Component Parts in PDF. No access codes are included.
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